Activities

Our schedule
Intro to Biotech Case study part 1 || Fruit DNA isolation (45 minutes) Genes in a Tube (45 minutes) ||  ||
 * > Day ||> Morning ||> Afternoon ||
 * Monday 6/24 || Boston ||  || Need to prep ||
 * Tuesday 6/25 || Museums ||  ||   ||
 * Wednesday 6/26 || Kim Intro ||  ||   ||
 * Thursday 6/27 || Intro
 * Thursday 6/27 || Intro
 * Friday 6/28 || Into to SynBio

Case study part 2 || Intro design project || Gels, buffer ||
 * Saturday 6/29 || Whale watch ||  ||   ||
 * Sunday 6/30 ||  ||   ||   ||
 * Monday 7/1 || Case study part 3

Work on Projects || Principles of gel electrophoresis (1 hour) ||  || Case study part 4 (role play) || DNA restriction fragments (gel) (1 hour) || Prep for PCR || work on projects ||  ||
 * Tuesday 7/2 || Bioinformatics (1 hour)
 * Wednesday 7/3 || PCR amplification of DNA (PCR 3 hrs + Gel 1 hour) || PCR amplification of DNA Continued
 * Thursday 7/4 ||  ||   ||   ||
 * Friday 7/5 || Work on projects || Plasmid mini prep || Prep Bacterial transformation ||
 * Saturday 7/6 ||  ||   ||   ||
 * Sunday 7/7 ||  ||   ||   ||
 * Monday 7/8 || Vertex Field trip || Bacterial transformation (90 minutes) || Human PCR ||
 * Tuesday 7/9 || Work on projects || Human PCR ||  ||
 * Wednesday 7/10 || Work on projects || Human PCR continued ||  ||
 * Thursday 7/11 || Work on projects || Work on projects ||  ||
 * Friday 7/12 || Presentations || Presentation ||  ||
 * Saturday 7/13 ||  ||   ||   ||

A case study introducing genetic engineering and synthetic biology
Please do not read ahead.

Power Points and videos:
DNA sequencing--Sanger Method (YouTube) Automated DNA sequencing (YouTube) PCR animation (YouTube) PCR lecture (YouTube) Jay D Keasling: Synthetic Biology Towards Biofuels (YouTube)

Required reading and watching
Synthetic Biology explained (YouTube) [|Decoding Synthetic Biology (YouTube)] [|The Promise and Perils of Synthetic Biology]

The Design Project
BioBuilder Design Assignment

Research topics (some suggestions)
Research a topic and prepare a PowerPoint. If possible try to relate it to your design project. Try searching for these terms with the phrases iGEM and/or synthetic biology Biopharmaceuticals Artemesinin RE coli Man made genome (Venter) Minimal cells Bacterial logic gates Bacterial computing Bacterial counting Artificial chloroplasts Cyanobacterial energy Biosensors and toxins/heavy metals Biosensors and landmines Biosynthetic biofuels Bacterial photography Rewriting the genetic code Mosquito control Quorum sensing Two component signaling system Therapeutics

Our lab experiments
( Link to BLASTN)